My current research work aims to characterize the adaptive immune response in chronic respiratory diseases, with a focus in Chronic Obstructive Pulmonary Disease (COPD), but also aiming at asthma, idiopathic pulmonary fibrosis (IPF) and allergic rhinitis, using cutting-edge methods based on immunohistochemistry and computerized image analysis. Proficient in conducting desk-based and field research. Developed several detailed protocols on research methods, data collection and analysis and published 20 scientific papers in well reputed journals. Diligent and driven with highly developed technical, organizational, presentation and analytical skills. Experienced in conceptualization, design, implementation, leading of research projects involving human beings, and hands-on skills in a number of lab methods, including ELISA, blood cell culture, flow cytometry, Luminex, real-time PCR and fluorescence microscopy. I plan to establish a career as a researcher in the area of infection immunology.

Male

18-11-1988

Brazilian

Malmö

Sweden

Experienced (Non-Manager)

Clinical Microbiology, Infectious Diseases, Public Health, Microbiology

Lund University

Jonas Erjefält

Lund

Sweden

Research Fellow

02-01-2022

01-31-2024

Jan 2019 – Dec 2021
University of Campinas, School of Medical Sciences, Center for Investigation in Pediatrics (CIPED)
Postdoctoral researcher
Funded by the São Paulo Research Foundation (FAPESP)
Research project: Innate and adaptive immune response to Pseudomonas aeruginosa and Nontuberculous Mycobacteria in cystic fibrosis lung infections (Leading investigator)
The research project aimed at setting a profile of the immune response of patients with cystic fibrosis (CF) to Pseudomonas aeruginosa, nontuberculous mycobacteria (NTM) and also M. tuberculosis (MTB) by using peripheral mononuclear blood cell (PBMC) culture and multiparametric flow cytometry. We found that CF patients vaccinated with BCG respond equally to MTB antigenic stimulation when compared to their non-CF counterparts, but no proliferative response was detected when their PBMC was stimulated with P. aeruginosa, raising concerns about bacterial or host immune factors that can possibly impair the response to this pathogen. This research yielded a publication in the Journal of Cystic Fibrosis, in parallel with three other publications in highly reputable journals.

Jan 2020 – Dec 2020
Rigshospitalet (Copenhagen University Hospital)
Guest Researcher
Funded by the São Paulo Research Foundation (FAPESP)
Research project: Early diagnosis and role of immunological memory in nontuberculous mycobacterial lung infections in patients with cystic fibrosis
Our aim was to compare the immune response to Mycobacterium abscessus complex (MABSC) between CF patients with and without chronic NTM infection, BCG-vaccinated and non-vaccinated healthy individuals. We found a similar pattern of immune response between CF patients with chronic NTM infection and BCG-vaccinated healthy individuals, both presenting a significantly higher T cell proliferative response to MABSC when compared to non-vaccinated controls, pointing to a potentially protective role of the BCG vaccine against MABSC and possibly other NTM. This research yielded a publication in Frontiers in Cellular and Infection Microbiology.

Sep 2018 – Jan 2019
Cactus Communications
Freelance editor
Publication support and academic editing in the field of medical sciences.

Aug – Dec 2021
Invitare Pesquisa Clínica (CRO)
Continued education on clinical research
São Paulo, Brazil

Sep 2014 – Aug 2018
University of Campinas
Ph.D, Health Sciences
Campinas, Brazil

Aug 2012 – Aug 2014
University of Campinas
Master of Science, Health
Campinas, Brazil

Mar 2007 – Dec 2011
Federal University of Alfenas
BSc. Degree, Biomedicine
Alfenas, Brazil

Portuguese

Native

English

Excellent

Swedish

Good

Spanish

Good

• Flow cytometry
• Cell culture
• ELISA
• Blood cell isolation, purification, and long-term preservation
• Immunohistochemistry
• Digital Image Analysis
• Multiplex Cytokine Analysis
• Chemiluminescence
• Real-time PCR
• Data storage and archive
• Statistical analysis
• Protocol writing

Published 20 papers in international journals as main author or coauthor;
Reviewed 38 scientific manuscripts for 21 different journals as a voluntary peer-reviewer;
Invited Professor of Scientific Writing at Centro Universitário Lusíada – Santos, Brazil (2019);
Contributed to the organization of five scientific events since 2010;
Worked part-time as a teaching assistant in 2013 and 2015, gaining important teaching skills;
Worked part-time as a teaching assistant in 2013 and 2015, gaining important teaching skills

Research position, Laboratory position

Clinical Microbiology, InfectiousDiseases, Microbiology

Associate Researcher; Senior Researcher; Assistant Researcher; Research Fellow

Permanent, Temporary/contract/project

Full-time

5

The Öresund region - Malmö and Copenhagen Metropolitan Area

02-01-2024

I expect to perform desk-based research assessing the interaction between infectious agent and immune system by using cutting-edge techniques such as cell culture, flow cytometry, immunohistochemistry, real-time PCR and spectrometry. A special interest is mapping the immune response to different infections in the tissue using multiplex fluorescence immunohistochemistry, which provide a picture of immunological events that actually happen in the body and are not always detected by blood-based studies. This can provide a solid foundation for research aiming at specific immunological pathways that can play a crucial role in the infection's development and reveal potential drug targets.