Technical skills
-Animal handling (cats, dogs, horses and cows).
-Aseptic techniques to take biological samples (e.g. blood, urine, skin).
-Environmental sampling from swine farms
-Aseptic in vitro microbiological techniques (e.g. spread plate method).
-Time-kill curve after adding antibiotics
-Antibiotic susceptibility test (MIC in broth and agar, Kirby-Bauer, Combination disk test, E-test) and minimum bactericidal concentration (MBC).
-Use of MALDI TOF-MS for bacterial identification
-Biofilm treatment with different types of stains (resazurin, crystal violet, live and dead stain).
-Work in lab with bacteria, viruses and phages (BSL2).
-Phage spot assay on double-layer agar
-Proteinaceous antimicrobial compound assay with proteinase K and/or trypsin digestion
-Colicin well diffusion assays
-Immunoassays (ELISA and Complement fixation tests).
-Gel electrophoresis.
-Use of UV-transilluminator and spectrophotometer
-Confocal and optical microscopy.
-preparation of different culture media (e.g. Blood agar, MSA, MCC3).
-DNA and RNA extraction using commercial kits and boiling method.
-DNA purification (enzymatic technique)
-Molecular tests (PCR, q-PCR).
-Use of alignment tools (e.g. BioEdit, MEGA, BLAST)
-Assembly of raw nucleotide data (e.g. SPAdes, DFAST)
-WGS data analysis (FASTA and FASTQ), and annotation through bioinformatic tools and web platforms (e.g. BAGEL4, BV-BRC bioinformatics, Islandviewer4, Proksee, PHASTER, Phigaro, ResFinder, Virsorter)
-Submitting bacterial genomes (WGS) to GenBank

Soft skills
-Time managing (e.g. submissions of multiple assignments at the same time).
-Flexibility (e.g. change schedule while I am making an experiment, going abroad).
-Critical and analytical thinking (e.g. scientific essays, conferences and dissertation for my university degrees).
-Confidence (e.g. presentations in front of lecturers and students).
-Effective communicator (international conferences, dissertations).
-Team working (e.g. work in pair and in group)

Female

01-01-1988

Italian

Torino

Italy

Clinical Microbiology, Infectious Diseases, Microbiology

APC Microbiome Ireland

Scanlan Pauline

Cork

Ireland

Post-doc Researcher

12-01-2022

12-31-2023

-01/04/2022-28/10/2022
Researcher at the Veterinary Epidemiological Observatory, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, Brescia (IT).
Main activities and responsibilities: research on zoonotic pathogens of public health interest in veterinary medicine. Surveillance of animal infectious diseases at regional and national level. Publication of research articles, abstracts and systematic reviews focussing on antimicrobial resistance (AMR) and antibiotic usage (AMU) in livestock.

-08/2015-07/2016:
Night vet, 'San Siro' Veterinary clinic, Milano (IT).
Main activities and responsibilities: veterinary emergency service.

-2018-2022
PhD, University of Turin, Department of Veterinary Sciences, Sector of Infectious Diseases.
PhD project: “Occurrence of Extended spectrum β-lactamases-producing Escherichia coli and Methicillin resistant Staphylococcus spp. in swine farms and association with sanitary and productive parameters”
Main activities: field work and data collection; laboratory work: laboratory preparation of broths, media and reagents, phenotypic bacterial identification using different types of broth and media (differential, selective and non-selective), use of MALDI TOF-MS for bacterial identification, antibiotic susceptibility test with Kirby-Bauer technique, Combination disk test and E-test, bacterial DNA extraction with boiling methods and commercial kits, application of molecular biology tools (multiplex and simplex PCR, traditional and Whole genome sequencing and phylogenetic analysis); use of alignment tools (BioEdit, Mega) use of assemblers (SPAdes), application of bioinformatics tools to analyse WGS outputs (nucleotideBLAST, PATRICbioinformatics, ResFinder, VirulenceFinder, MLST, CHtyper, PlasmidFinder, pMLST, SeroTypeFinder, MGE, PathogenFinder), data management (goeBURST algorithm), and statistical analysis.
-2017-2018:
MSc Clinical Microbiology, School of Health and Life Sciences, Glasgow Caledonian University (UK).
Main modules: Medical microbiology, Re-emerging infectious diseases, Microbial genetics, Applied molecular microbiology, Microbial pathogenicity, Case studies in Infectious diseases, Research project on “in vitro Staphylococcus aureus susceptibility against antibiotic oxacillin”.
Main activities during the Research project: testing the effect of the antibiotic oxacillin on SAUR02, S. aureus clinical isolate. Analysis of in vitro activity of the antibiotic on planktonic and biofilm cells. Analysis of toxins genes, sea, seb, sec, sed, see and tst, present in the clinical isolate DNA, through PCR. Test of toxins genes expression under sub-inhibitory concentrations of oxacillin, through q-PCR. Perform immunoassays using ELISA tests (assay based on the detection of the link between antibody and related antigen), to assess variations in toxins production in presence and in absence of sub-inhibitory concentrations of oxacillin.

-23/07/2014:
Graduation, Faculty of Veterinary Medicine, University of Milan (IT).
Name of the course of study: Veterinary Medicine
Final degree mark: 104/110.
Dissertation/thesis title: Lyme Borreliosis Complex and Lyme Disease: is there anything new?

English

Excellent

Italian

Native

French

Basic

-Microsoft Office™ package (e.g. Power point presentations, Publisher posters, Word for Lab reports and Scientific Publications, Excel for data management)
-satisfactory knowledge of the bioinformatics software BioEdit and MEGA, acquired through work and training at University of Turin (Italy).
-SPSS Statistics V 23.0 (for statistical analysis)
-Scientific literature review (e.g. PubMed, Google Scholar)
-GraphPad Prism 7.0
-creation of the personal website through “Wix platform”
-use of different web platforms and tools to analyse WGS data (raw and assembled) (e.g. Proksee)

Training position, Research position, Clinical position, Laboratory position, ESCMID observership

Clinical Microbiology, InfectiousDiseases, Microbiology

Researcher

Permanent, Temporary/contract/project, Short visit

Full-time

7

Italy

04-01-2024

Researcher